Nettet14. jul. 2024 · Live imaging is a key tool in understanding the organization and function of cells, tissues and organisms, since it allows the visualization of dynamic processes within their native environment. However, in practice, the live-imaging of multi-layered tissues with different cell types often poses major challenges. NettetThe best performing probe, 5-HMSiR-Hoechst, contains the 5′-regioisomer, shows ∼400-fold fluorescence increase upon DNA binding and is compatible with wash-free single …
Live Imaging of Tumor Initiation in Zebrafish Larvae Reveals a …
NettetHoechst (or NucBlue Live) would not be a good choice, because the excitation of Hoechst (usually 408 on a confocal, broader on an epi scope) will induce phototoxic … Nettet(for excitation of QDot, wtGFP, Hoechst Blue and DAPI) 9 . Epi White Light Excitation . Turns “ON” or “OFF” the visible overhead white lighting (for focusing in live preview and opaque colony plates) 10 . UV Transilluminator . Controls the intensity of the 300 nm UV transilluminator; (use high intensities for imaging and lower ... bala guaraná
Endocytic internalization mechanism of bioactive antibacterial ...
NettetI've successfully used Hoechst 33342 for live imaging of random cell migration in different types of breast cancer cell lines. Staining with a low concentration (100 ng/ml) for 45 … NettetDyes for live cell staining of organelles are available in a broad spectrum of colors. Find out about dyes for specific organelles: Actin filament staining (red, ab112127) in Hydractinia, a colonial marine hydroid. Nuclei are shown in blue (Hoechst) for counterstaining. Image courtesy of an Abreview. Live cell tracking NettetHoechst dyes are generally preferred for live cell staining over DAPI because they are less toxic and more cell permeant. Biotium offers both Hoechst 33342 and Hoechst … balaguer alguaire